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November 13 to 16, 2017

 

Products Range

NEW-Resist Range

Oxa-48

KPC

RESIST-3 O.O.K.

RESIST-3 O.K.N.

Immunochromatographic Range

Pathogens detection

40/41 Adenovirus

Adenovirus

Clostridium difficile

Cryptosporidium & Giardia

Cryptosporidium

Escherichia coli O157

Rotavirus & 40/41 Adenovirus

Giardia

Helicobacter pylori

Influenza A & B viruses

Legionella

Respiratory Adenovirus

Rotavirus & Adenovirus

Rotavirus

Respiratory Syncytial Virus (RSV)

Streptococcus (group A)

Trypanosoma brucei gambiense (HAT)

Quatro-VeT (Veterinary)

Molecular Range

Leishmania

Pseudomonas aeruginosa

Scientific partners

PARADIS (Biowin)

CCH Fever (EC-FP7)

Chips 4 life (EC-FP7)

Nidiag (EC-FP7)

AquaVir (FP7)

Nareb (FP7)

Euroleish (H2020-MSCA-ITN)

ebolaMoDRAD (IMI-2)

 

Products  >  Molecular-Field  >  Leishmania  >  FAQ

LEISHMANIA SPP. - FAQ

Is the Oligochromatographic test (Leishmania OligoC-Test   and  T. cruzi OligoC-Test)  still valid even if the amplification mix arrives defrosted?

The Oligochromatographic kit (including Ampli-Mix and Ampli-Control) is always sent to room temperature. The reagents to store at -20 °C are kept at -20°C in the storage in production and they are put in the kit just before sending.
Upon receipt of the package by the customer, the small box named "Ampli-Reagents " (inside the OligoC-Test kit) containing the amplification mix (Ampli-Mix) and the positive control template (Ampli-Control) should be placed at -20°C upon receipt. We guarantee the stability of these reagents (the mix and the positive control) at room temperature for the time of transport.

Is the buffer the same for all Coris BioConcept kits? If I need some more for my tests, may I use the one from another kit?

According to the "Instructions For Use", it is not allowed to exchange buffer between kits. If you have volume problems, please contact your distributor who is able to provide additional vials. Nevertheless, the buffer is the same within one product of different lot numbers (e.g. Rota-Strip buffers exchange between different lots will not give rise to any problems on the strips).

At present, 5 different kinds of buffers are used in Coris’products, i.e., Saline Buffer (for Influ-A Respi-Strip, Influ –A&B Respi-Strip), Extraction Buffer (for RSV-Strip), HC Dilution buffer (for Adeno Respi-Strip, Combi-Strip, Pylori-Strip, GastroVir-Strip), HydroK buffer (for dry swab Test restricted to Adeno Respi-Strip) and Sample Dilution Buffer for all the other products. Except the detection kits based on PCR method (T.cruzi OligoC-Test and Leishmania  OligoC-Test) have their own running buffer.

Regarding the GastroVir Kit we received some particular comments claiming that the Control Line was too faint. The buffer has been improved to allow a better migration of the latex microspheres leading to a better and a more homogeneous signal. This improvement does not affect any characteristics of the kit (i.e. specificity and sensitivity).

When dipping the stick in diluted sample, the level of sample passes beyond the limit indicated by the arrows at the bottom of the stick. Is there any consequences on the test reactivity?

The red arrow indicates the limit that could not be exceeded beyond during any incubation.
It could happen that in some cases operator exceeds the indicated limit line by using different but routinely used tubes in laboratories. However, it's still possible to security a validate test as long as the incubation level is still below 1cm (i.e. the green line indicated on the figure) while the gold conjugate is placed at 2cm from the bottom of the stick.

Why is stability relatively short after opening of bottle containing strips?

The product is stable during at least 15 weeks after first opening. 15 weeks is quite high: that means at least 2 tests can be performed per week. Stability depends on the storage conditions.
Immunochromatographic test uses nitrocellulose support which is sensitive to humidity.
That's why the desiccant is recommended to be kept in the tube and in the cap.

In each case, there is an internal control (upper line). This internal control will appear if the test is running well. However the test could be used after 15 or 20 weeks and the result can be valid if the control line appears.

How to use correctly Coris BioConcept's kits?

  • Always store the reagents at the indicated storage temperature : between 4 and 30°C for all kits except for Oligochromatographic kits which contain Ampli-Reagents that requires to store at –20°C
  • Always use gloves manipulate kit components and samples
  • Always use the provided (Extraction, Dilution, …) buffer even for the sample already diluted (e.g. in a transport medium)
  • Always ensure that the solution sample – buffer is homogenous before testing
  • Always ensure the sample - buffer immerse below the limit line marked by red arrow on the strip to avoid colloidal gold conjugate dilution in the buffer
  • Always read the results when the stick is wet (between 10 and 15 minutes depending on the test)
  • After opening of the tube which contains the strips, quality is guaranteed during 15 weeks
  • Always close the tube after every use to avoid that strips become wet. Always leave the desiccant inside the tube. If storing the strips' tube at 4°C, always ensure to warm up to room temperature before proceeding with the test

How shall we use positive controls with our kits?

The positive control should be used diluted with the provided buffer of the respective kit and plunge directly the strip into the dilution. The mixture should then be well homogenized. Use the mixture accordingly to kit instructions.

How many tests can be performed with a positive control?

It depends on positive controls which are proposed  in freeze-dried form or in liquid form.
The dilution step of positive control will determine the number of possible tests. Please reference it to the use's instruction of each positive control.

The bottom of the nitrocellulose of the strip shows a pinkish aspect. Is it normal?

No. Maybe the sticker (with arrows) covering the bottom of the nitrocellulose is peeled off. In this case, the test is invalidated. Please perform the test with another strip.

Is it a problem if the test has "run" more than the recommended time?

Yes. It might cause the appearance of false positive signal on test.

The signal of the test line is very weak in comparison with the control line. Do I have to consider that the result is positive?

The control line is aimed to ensure the user that the chromatography is performed up to the top of the strip. It does not give any indication on the diagnostic result. Whenever a signal is observed on the test line (weak or strong), it means that the sample is positive for the pathogen of interest.

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